Seagrass meadows provide important ecosystem services by stabilizing the seabed, protecting shorelines, sequestering carbon and providing food and habitat for marine life. Along the Adelaide coastline, more than 6,200 hectares of seagrass were lost since 1949, mainly due to poor water quality from industrial discharges, stormwater run-off and sewage discharges causing eutrophication. Despite reduced anthropogenic inputs and ongoing restoration efforts, recovery remains limited in near-shore high-energy environments where hydrodynamic forces dislodge newly planted seedlings. A key challenge in restoration is to maximize seedling survival by enhancing root development for better anchoring in the sediment. To enhance Posidonia establishment, we explore the effects of four plant growth regulators on root development and survival of P. angustifolia seedlings. Laboratory experiments monitored seedlings treated with different combinations and concentrations of naphthyl acetic acid, indole-3-butyric acid, 6-(γ,γ-dimethylallylamino)purine, and 6-benzylaminopurine. Seedlings were observed during early development, and at three, four and six months. The project also aims at optimizing practical restoration by assessing the feasibility of hormone applications under different conditions including delayed hormone application, delayed planting and different treatment applications, while also exploring seedling storage for delayed planting to combat winter storms. Findings will improve restoration success and contribute to developing scalable, science-based strategies.