The Australian hybrid abalone is a cross between greenlip abalone (Haliotis laevigata) and blacklip abalone (Haliotis rubra) and is favoured for their superior performance. However, not many studies have been conducted on the effect of functional feed additives in their diets and their effect on modulating the intestinal microbiome. Hence, this study was conducted to assess the effect of NovaqPro®, a microbial biomass supplement on the growth performance and intestinal microbiome of Australian hybrid abalone. An experimental diet consisting of 10% NovacqPro® and one without supplementation (control) were fed to triplicate tanks for 98 days under controlled conditions. At the end of the experiment, abalone intestines (n=6/tank) and faeces were collected from each tank, along with eDNA samples of tank water. DNA was extracted from all collected samples. Variations in global assemblages of bacterial communities were identified by 16S rRNA gene next-generation sequencing analysis. The growth performance of abalone fed NovacqPro® was significantly higher than the control-fed abalone (p ≥0.05). Moreover, the preliminary evidence suggests that there is a shift in the intestinal microbiome of the abalone fed the experimental diets and it can be assumed that NovacqPro® positively modulates the intestinal flora of Australian hybrid abalone.