Seagrasses are some of the most important coastal marine habitats yet have seen significant global losses to date prompting the development and increased demand for active seagrass restoration. Although a relatively new field of research, seagrass restoration is advancing at a rapid rate with new approaches and tools being developed for different species. However, with ambitious restoration targets set globally, there are still several limitations common to many restoration programs, including the need to source material from donor meadows and the low survival of seeds sowed directly into restoration sites. The emerging practice of nursery cultivated plant material from seed has become an increasing prospectus for seagrass restoration. Nursery based experiments were conducted in Wales, UK and Victoria, Australia investigating seed viability and germination triggers for Zosteraceae species (Zostera marina and Zostera muelleri). Aims of this research are to investigate the use of seagrass seed characteristics as seed viability indictors, alongside investigating the effect of germination triggers on germination success. Here, we present the findings of this research to support the provisioning of nursery-cultivated plants and enhance seagrass restoration efforts by identifying how seed characteristics translate to germination success.