The Great Barrier Reef (GBR) holds immeasurable cultural value to more than 70 groups of First Nations peoples, but like all coral reef ecosystems globally the GBR is threatened by climate change. Cryopreservation and biobanking (the storage of frozen, living cells in liquid nitrogen) can be used to secure the genetics of threatened species, but involves the culturally sensitive practice of relocating parts of Country (the biobanked samples) to specialised facilities for secure storage. An inaugural on-Country coral biobanking project occurred in 2022 on Konomie (North Keppel Island) involving partners from Taronga Conservation Society, the Australian Institute of Marine Science, and Woppaburra Traditional Custodians. During this project and a subsequent Biobanking Cultural Safety event held on Cammeraygal Country at Taronga in September 2023, we discussed cultural matters related to biobanking and measures that could be taken to ensure that the enduring link to Country of these samples is maintained in perpetuity. These discussions and the outcomes from the workshop will form the basis of a best-practice approach to biobanking activities in other regions of the GBR, in partnership with the Traditional Custodians of those sea Countries.